The aim of this study was to evaluate the protective effect of broccoli sprout extract (BSE) against ovarian and renal toxicity induced by methotrexate (MTX) in female albino rats. Methotrexate, an antimetabolite frequently utilized in cancer and autoimmune diseases, has been linked to nephrotoxicity and primary ovarian insufficiency through the induction of oxidative stress and apoptosis. Because of their rich content in glucoraphanin and sulforaphane, two powerful antioxidants, broccoli sprouts were chosen. This study comprised six groups of Thirty female albino rat (180–225 g) (n = 5): (A) Control; (B) MTX, 0.15 mg/kg/week; (C) BSE, 5 mg/kg/day; (D) BSE, 7.5 mg/kg/day; and (E) MTX + BSE, at doses of 5 and 7.5 mg/kg respectively. All treatments were administered orally. The administration of MTX induced severe ovarian histological injuries, indicated by significantly reduced number of follicles, blood vessels rupture, inflammatory cell infiltration and partly fibrosis. Administration of MTX significantly increased the levels of urea and creatinine in renal tissues and induced glomerular damage such as congestion, necrosis, and cellular vacuolation. Consistent with this, these histopathological changes were prevented by simultaneous administration of BSE which appears to restore folliculogenesis and renal function parameters. In conclusion, the findings indicate that BSE strongly protects against MTX toxicity through a mechanism involving enhancement of antioxidant homeostasis. Thus, to summarise, broccoli sprout extract is a effective naturl adjuvant for mencerpt treatment in rescinding side effects on female reproductive and renal systems.

Syzygium polyanthum (Wight) Walp, known as Indonesian bay leaf, is a plant widely used by many ethnic groups in Southeast Asia as a traditional medicinal plant. This study aims to reveal the regenerative effects of bay leaf extract on histopathological damage to the kidneys and pancreas of hyperglycemic mice induced by alloxan. The test animals were divided into 5 groups, each consisting of 5 mice. The first group was only given food and water as a control. The second group was induced by alloxan as a negative control. Groups 3, 4, and 5 were induced by alloxan and bay leaf extract at doses of 150, 250, and 350 mg/kgBW, respectively. Alloxan induction was carried out once every two days for 6 days, while bay leaf extract was given after induction once daily for 14 days. Blood glucose levels and kidney and pancreas damage scores of mice were examined on days 1, 10, and 25. The results of the experiment showed that blood glucose levels, renal dan pancreatic histopathology damage in mice decreased after being given the extract for 14 days. Thus, it can be concluded that bay leaf extract has regenerative properties against renal dan pancreatic histopathological damage in alloxan-induced hyperglycemic mice.