The objective research works on cold and ready-to-eat meat products from local markets and how they are affected by external factors and the transmission of microorganisms to them and their reproduction despite their preservation, despite the preservation of these meats in consumers' refrigerators, and how some of these microbes are pathogenic or cause diseases by excreting toxins. The result of our research centers on displaying the total number of bacteria in all samples, identifying the strain contained in these ready-to-eat meats, and comparing them with the permissible limits imposed by most food and drug authorities in the world to preserve the safety of consumers. Also, we test the microbes' sensitivity to antibiotics. The types of bacteria were identified through approved protocols. The total count of bacteria was not in the range of the FDA limits rate, and we found some strains that should not be in the product as E. coli and Salmonella. spp. These strains can cause severe gastrointestinal tract diseases. The results of the Antibiotic test were positive. Only two of the 30 species we identified were resistant to all the antibiotics we used. They were S. aureus. The microbiological quality of cold meats in Jeddah, Saudi Arabia, is barely within the limits of the FDA. In terms of coliforms, Staphylococcus, and streptococci. So, all the food handlers should receive training to increase the level of hygiene in the supermarkets in Jeddah, Saudi Arabia.

The work is devoted to statistical methods for determining the comparative sensitivity of sterility analyzers with a binary scale: 1/0, +/-. The paper considers both traditional statistical test methods namely the direct binomial test, the McNemar test, and the new, highly sensitive Yefimov Method, and Pearson-Yefimov statistical test. First, the comparative effectiveness of statistical methods for assessing the sensitivity of testers is demonstrated on a specially developed model system (the Test Bench), and then on real experimental data. The data were obtained during the validation of a new tester and a new method for analyzing samples for sterility. The new method is compared with the old one, the certified method. The sensitivity of both new and old methods (testers) was already determined earlier by a direct non-statistical method, which allowed us to compare the results of the two approaches. The factors influencing the efficiency of statistical tests are revealed and described.

Background: Covid-19 vaccine is an immunization method used to reduce coronavirus incidence but lately caused life-threatening events such as thrombosis with thrombocytopenia syndrome. Methods: This is a case-control study conducted at Shendi town to evaluate platelet parameters and fibrinogen levels in vaccinated healthy individuals With Covid-19 vaccines between Augusts to November 2021. A total of (100) vaccinated healthy individual With the Covid-19 vaccine was enrolled in the study as test groups, compared with (50) healthy volunteers as a control group. Venous blood samples were transferred into Trisodium citrate and EDTA anticoagulant. Data was collected using a questionnaire and the (SPSS) version (22) program was used for data analysis. Results: The study revealed that the vaccinated healthy individuals were; (50%) male and (50%) female. The platelets parameter and fibrinogen indicated the mean values of platelets count, mean platelets volume, platelets distribution width, platelet crit, and fibrinogen, in Janssen vaccine groups, were (251.14 109\L), (8.39 fl), (15.6), (0.203%), and (158 mg\dl) respectively. The results study revealed the mean of PLT, MPV, PDW, PCT, and Fibrinogen, in Astrazeneca vaccine groups, were (272.08 109\L), (8.13fl), (15.56), (0.2211%), (159 mg/dl) respectively. Also explained the mean of PLT, MPV, PDW, PCT and Fibrinogen, in Astrazeneca vaccine groups were (272.08 109\L), (8.13fl), (15.56), (0.2211%), (159 mg\dl) respectively and in Janssen vaccine groups the mean values of PLT, MPV, PDW, PCT, Fibrinogen were ( 251.14 109\L), (8.39fl), (15.63), (0.2034%), (158 mg\dl) respectively. Conclusions: Covid-19 vaccines are responsible for significant changes in fibrinogen level in both AstraZeneca and Janssen vaccine groups.

Quorum sensing (QS) is cell to cell signaling mechanism that enables bacteria collectively control gene expression in response to their population density, or it is bacterial communicate with one another using chemical signals. There are three types of signaling molecules: Acyl-homoserinelactonase (AHLs), Auto-inducer peptides (AIPs) and Autoinducer-2 (AI-2). Q.S was first observed and described in bioluminescence bacterium V.fischeri.In V. fischeri, several kinds of quorum sensing system were identified. At first, lux system was found and regulates the luciferase operon and light production. LuxIwas isolated as an AHL synthase while LuxR was isolated as a transcriptional activator of the luciferase operon. At low cell density, LuxOrepresses LitR, which is a positive regulator of the expression of LuxR. Quorum quenching (QQ) refers to the mechanism by which bacterial communication can be interrupted, or it is the process of preventing QS by disrupting the signaling.The first major QS-disrupting strategy that has been studied is the interference with the detection of the AIs and the second one is the inactivation/degradations of the signal molecules.

The study was carried out to detection of Toxoplasma gondii in (135) women patients with repeated abortion with age ranged 18-43 years who attended two teaching hospitals in Baghdad. The diagnosis is done by Immunochromatography and ELISA methods. a blood samples was taken from each patients as well as other(30)healthy control matching in age. The study included measurement the concentration of Interleukin-6, Interleukin-17, Dopamine and Complement in sera of patients and control .The result indicated presence the of anti-Toxoplasma IgG in 26 cases a, Anti-toxoplasma IgM in 14 cases and 10 in both out of 135 cases of aborted women with Toxoplasma in immunochromatography methods while the level of IgM 1.57 IU/ml and IgG 142.73. Also, the result indicated significant increasing levels of IL-6 and IL-17, Complement and Dopamine in patients sera in comparison with healthy control.

The present study was undertaken to investigate the learning and memory enhancing activity of Cucurbita pepo seed extract in rats by using the ethanol induced cognitive impairment and its effects were examined by using 8-radial arm maze (8-RAM) test. For radial arm maze test all groups showed significantly (P value is< 0.0001& < 0.001) decreased in the time taken to reach the paired arm and number of entries in baited arm and non-baited arm as compared to ethanol inducing group. Therefore seed extracts of Cucurbita pepo exhibited significantly learning and memory activity in Alzheimer’s disease.

We were tasked with testing a multicomponent aqueous solution for injection used in veterinary medicine. The composition of the solution included the following components: folic acid, tryptophan, nicotinic acid, thiamine hydrochloride, ascorbic acid, sodium acetate, and benzyl alcohol. The composition indicates that the drug is intended to stimulate the vital activity of the body. It was required to determine the activity and stability of four components: folic acid, tryptophan, nicotinic acid, and thiamine hydrochloride. The complexity of the simultaneous determination of these components was that they differ greatly in their acid-base properties and solubility in water. We managed to solve the problem using an Agilent HPLC/DAD/MS instrument and selected the parameters to determine all four components together reliably and accurately or each separately by one method.