Abstract: Imidacloprid is an insecticide used widely in the United States and Canada to kill insects that cause harm in farms and affect wide range of vegetables, seeds and fruits crops. In this review we use many evidences to suggest the responsibility of chronic exposure to Imidacloprid in development of Creutzfeldt –Jakob disease by causing modification phosphatidylinositol anchor on the prion protein. Excess use of these insecticide in Canada and US lead to occurrence of most cases of CJD in both countries. Experimental work needed to confirm this hypothesis.