Abstract: Back ground: The goal of this cross-sectional descriptive study was to isolate and identify several bacterial species in mobile phones among hospital staff at Almak Nimer University Hospital and Shendi Teaching Hospital in Shendi town, River Nile State, North of Sudan. Between December 2019 and November 2020, the research was carried out. Methodology: One hundred (100) swabs were randomly taken from the phones of male and female medical staff volunteers from Almak Nimer University Hospital and Shendi Teaching Hospital, Blood agar and MacConkey agar were used to grow the specimens. Finally, colonial morphology, gram stain, and biochemical tests were used to identify the isolated bacteria. Results: Eighty-seven (87) mobile phones were discovered to be cultured positive in the research (87 %). The isolated organisms were: S. aureus (28; 32%), Coagulase gram negative staphylococci (26; 30%), Bacillus spp (16; 18%), P. aeruginosa (7; 8%), K. pneumonia (5; 6%), E. coli (4; 4%) and Diphteroid (1; 1%). Laboratory technicians (93%) were the most likely to be contaminated, followed by nurses (88%), physicians (80%), and pharmacists (50%). In terms of gender contamination, females account for 87%, while males account for (86 %). Conclusion: The results of this study showed that medical staff's phones might be a source of nosocomial infection.