Abstract: The aim of study is to determine the activity of aqueous extract of Camellia Sinensis (Green tea) on different types of bacteria isolated from 191 pregnant women attended to Shendi locality hospitals, suffering from urinary tract infection between February 2015 to February 2017 following informed consent. One hundred thirty seven bacteria were isolated, different bacteria with different Gram reactivity was isolated then identified biochemically, in vitro sensitivity testing using well diffusion technique against aqueous green tea extract was done. The largest zone of inhibition appeared in Gram positive bacteria Enterococcus fecalis (17.6 ± 1.9 mm).the concentration of green tea aqueous extract able to suppress the growth of bacteria was 48.6 milligram per ml against Staphylococcus aureus and 280.2 milligram per ml against Escherchia coli.