Background: Occurance of black discolouration of teeth is very common and a clinical challenge in the present day dentistry. Stains associated with chromogenic bacteria have a high rate of recurrence even after thorough scaling and polishing procedures. The mechanism of stain formation is still not understood clearly though its chemical composition is mainly iron suiphide and bacteria like actinomyces have been identified in plaque samples of these patients which have the ability to form iron sulphides. The iron content of saliva is known to increase in iron deficiency anemia and iron overload. Iron deficiency anemia is also associated with low hemglobin levels. Aim: To determine the hemoglobins levels in patients with and without black chromogenic stains. Methodology: A total of 40 patients of age group 15-40 years were selected and divided in to two groups. Group ‘A’ (Test group) consisted of 20 patients with black stains on atleast 8 tooth. Group “B” (Control group) consisted of 20 patients without any stains on the tooth surfaces. The individuals were screened for Lobenes stain index, Oral hygiene index and haemoglobin levels. The scores were analysed using chi –square tests. Results: It was observed that there was no significant difference between the haemoglobin levels of patients with and without stains. The oral hygiene scores were found to be significantly higher in subjects with stains compared to controls. Conclusion: The study suggests that the presence of stains were not associated with the reduced haemoglobin levels. The chromogenic black stains were more prevalent among subjects with good oral hygiene.