This work was aimed at determining the antioxidant activity of an ethanol extract of Tetrapleura tetraptera fruits in rats administered DMH. Group 1 (positive control) contains rats without the administration of DMH, extract, or CMC. Group 2 (negative control) received DMH, 1 ml of 1% CMC, and no extract. Group 3 received a low dose of the ethanol extract of Tetrapleura tetraptera fruits (100mg/kg) and DMH. Group 4 received a high dose of the ethanol extract of Tetrapleura tetraptera (200mg/kg) and DMH. The DMH was administered once a week for four consecutive weeks, while the ethanol extract of Tetrapleura tetraptera was administered daily for four consecutive weeks. The result showed that there was a significant increase in the activities of SOD, CAT, and GST in rats administered an extract of Tetrapleura tetraptera fruits compared to the negative control group. In general, the introduction of Tetrapleura tetraptera to the rats caused an increase in the activity of the antioxidants compared to the negative control. The histopathological profile, however, showed that the DMH did not affect the liver, colon, and small intestine morphology. It could thus be concluded that the ethanol extract of Tetrapleura tetraptera fruit has potent antioxidant activity.

Squid ink is known to contain many bioactive properties such as antioxidant, anticancer activity, antimicrobial activity, and anti-inflammatory activity. Through its antioxidant properties squid was suggested to have therapeutic potential for sexual function disorders. This study is an attempt to evaluate whether squid ink has therapeutic benefits against age-related decreases in sexual arousal in D-galactose-induced aging male mice. Four groups of male mice were treated as follows. Group-1 received only standar food but no D-galactose induction nor squid ink given. Group-2 received D-galactose induction but not squid ink. Group-3 and group-4 received D-galactose induction and squid ink at the dose of 40 and 100 ml/kg body weight respectively. After 35 days of treatment, the test mice were mated with estrous virgin females to determine their levels of sexual arousal. The results, Loligo squid ink extract at a dose of 100 ml/kg body weight significantly enhances sexual arousal parameter values in the D-galactose-induced aging mouse model. However, the recovery rate did not achieve nor surpassed the parameter values of sexual arousal in normal mice. It suggests that Loligo squid ink has the potential to prevent damage to sexual function in males but is less effective in restoring it.