The effect of Indocid® was determined using thirty (30) albino rats of mixed sex, aged 3 months weighing 110-200g. They were acclimatized to their environment for 2 weeks during which they were fed with standard pelleted feed and water. The study is divided into acute toxicity testing and main experimental phase. The acute toxicity testing for the determination of the median lethal dose was ascertained using 12 rats divided into groups K, L, M. The main experiment using 18 rats divided into groups A, B, C, D, E and F of three (3) rats in a group in different cages, cage A served as control. The rats were orally administered graded doses of Indocid dissolved in water once daily along with their feed for 30 days. Cages B-F received 3.0, 2.5, 2.0, 1.5 and 1.0 mg/kg respectively and were closely observed. At day 31, the rats were randomly selected, sacrificed, the stomach, and small intestine were excised, examined grossly and subsequently processed for light microscopy. Grossly the organs and tissue appeared to have several perforations especially evident in the small intestine. Further histological examination revealed erosion of the stomach and intestine. This result forms the basis for further research into the danger posed to indiscriminate users of Indocid®.

Background: Urinary tract infections attack about 150 million people each year globally and nowadays there is a high emergence of antibiotic resistance strains among E. coli species that cause UTI. The objective of this study was to detect Aminoglycosides resistance among E. Coli strains isolated from patients with urinary tract infection. Method: A total of 70 urine specimens were collected from patient with symptoms of urinary tract infection in sterile urine containers, and then inoculated in CLED media, incubated at 37c for 24 hours. E. Coli species were identified according to their colonial morphology, indirect gram staining reaction, and biochemical tests. Identified species were tested for antimicrobial susceptibility against the following aminoglycosides: Amikacin, Kanamycin, Streptomycin, Gentamicin, and Tobramycin using Kirby-bauer disc diffusion method. Results: The study revealed that 55.7% of urinary tract infections were caused by E. Coli. The study also showed that 12.8% of isolated E. Coli species were resistant to Amikacin, 28.2% to Gentamicin, 43.6% to Streptomycin, 48.7% to Kanamycin, and 53.8% to Tobramycin. Conclusion: The study concluded that the overall resistance of isolated E. coli species to aminoglycosides was 37.4% the most powerful Aminoglycoside against E. Coli species was Amikacin.