Background: Nanoparticles, due to their small size and large surface area, have unique physicochemical properties that make them attractive for a wide range of industrial, medical, agricultural application. Objective: To prepare a novel nanoparticles from olive leaf extract and copper oxide and test its physiological effect of strawberry plants. Materials and Methods: Olive leaves extract was prepared according to a certain protocol, and the green nanoparticles were synthesized by coordination this extract with copper oxide. Characterization was done using many techniques such as FTIR, UV-Vis, XRD to identify the new copper nanoparticles. Different concentrations were prepared of CuONPs (10%, 20%, and 30%). Results: The FTIR spectrum shows peaks which confirm the coordination of olive leaves extract with the copper ion, while UV-Vis spectrum shows peaksthat confirm the formation of copper nanoparticles, the SEM shows the homogeneity of the nanoparticle that was confirmed by XRD spectrum and dataUsing30% (C3) give best seedling leaf length, and number of fruitswhile number of leaves are best in 20% (C2) compared to other concentrations. While the highest value in the number of flowers in treatmentof bio fertilizer. Best concentration of DNA appears in C3 (134.7) while best DNA purity is found on C2 (20%). The chlorophyll was also measured using SPADwhich show optimum with C3 (30%). Conclusions: Using30% (C3) give best seedling leaf length, number of fruits and chlorophyll while number of leaves are best in 20% (C2).

Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Sensitive, specific, and quick detection of FMD virus antigens and antibodies is an essential corner stone diagnostic tool at each tier of control strategy. Continued development of molecular and genetic technologies combined with novel analytical techniques will benefit many areas of FMD research and understanding. Genome sequencing provides excellent insight into the evolutionary and epidemiological dynamics of such viruses. The advent of next generation sequencing (NGS) technologies enables evolutionary studies of the entire viral swarm. Sequencing technologies have advanced uses of viral genomic data; which helps to understand the global distribution and Trans-boundary movements of Foot-and-mouth virus disease. Also sequencing information can be used to understand antigenic change within virus strains; therefore, the aim of this review is to assess various diagnostic techniques including conventional and advanced techniques both from agent and host response sides. Among the agent side virus isolation, RT-PCR, antigen ELISA and immune-chromatography strips are commonly used diagnostic tests. Whereas from the host side: infrared thermo gram, antibody ELISA and Virus neutralization are some mentions. Owing to current technological advancement: DNA sequencing and microarray, expressing a luciferase reporter and quantitative proteomics were reviewed. Therefore, potential confirmatory diagnostic test should be in combination with each other.