The present study was conducted to detect the contamination levels of some mycotoxin (Afla, Orra, and T2 toxins) in broiler feed by using the ELISA test. Twenty feed samples (500gm/ton for each sample) were collected randomly from broiler flocks feeds which are located in Misan province. The present study was conducted to detect the contamination levels of some mycotoxin (Afla, Orra, and T2 toxins) in broiler feed by using the ELISA test. Twenty feed samples (500gm/ton for each sample) were collected randomly from broilers flock feeds which are located in Misan province. The results revealed aflatoxin contamination in 15 (75%) and only (5) samples were free of aflatoxin. The levels of contamination ranged from 0.1 to 28.5 ppb, and only three (15%) of the aflatoxin-contaminated samples exceeded the US-FDA allowed limits. In terms of Orcha toxins, the findings of the examination revealed that contamination occurred in 7(35%) samples out of a total of 20, while 13 samples were clear of Orcha toxins, with contamination levels ranging from (o.4-34.4) ppb. The levels of ochratoxin in broiler feed did not exceed the European Commission's permissible limits, while T2 toxins were found in 8(40 %) samples and (12) samples were free of T2 toxins, with contamination levels ranging from (3.4-158.3) ppr, and the amount of T2 toxin in (2) samples (10%) exceeded the permissible limits.