Due to the recent increase in bacterial resistance to most antibiotics, alternative solutions must be found to reduce infection. Therefore, many recent studies have focused on plant extracts to assess their antimicrobial potential. The objective of this study was to detect the active constituents in Origanum majorana and evaluate its antibacterial activity against Staphylococcus aureus, Streptococcus pneumonia, and Escherichia coli. Origanum majorana is a medicinal herb rich in phytochemicals like thymol and carvacrol, traditionally used for treating respiratory and inflammatory conditions. Its essential oils have shown promising antibacterial effects in earlier studies. Plant leaves were collected, dried, and extracted using Soxhlet with ethanol, and active compounds were identified by GC-MS. Antibacterial activity was tested using the agar diffusion method against selected bacterial strains. The extract showed concentration-dependent inhibition, with the highest effect on Staphylococcus aureus (3.0 mm). Bioactive compounds such as Linalool, α-Terpineol, and n-Hexadecanoic acid were identified, supporting the plant's antimicrobial potential. These findings confirm that Origanum majorana possesses promising natural antibacterial activity, and suggest its potential as a source for developing alternative therapeutic agents.