The aim of this study was to evaluate the effect of CL, ovary weight and ovary size, on follicular population, oocytes recovery, oocytes grades, oocytes index and cleavage rate on Indian buffalos. A total of 296 buffalo ovaries were collected from slaughterhouse and transported to laboratory for determination of ovaries weight, length, width, thickness, follicular population and oocytes grades, oocytes index and cleavage rate. The results obtained revealed that the number of small and large follicles were significantly higher in ovaries having weight more than 5g as compared to ovaries weight between 3-5g and less than 3g. The number of small follicles in large size ovaries was significantly higher as compared to small size ovaries. The mean number of oocytes recovery, number of oocytes for IVEP and number of cleavage, obtained from ovaries with CL was lower than that of ovaries without CL. No significant difference observed between presence and absence of CL, follicular population and oocytes grades.