The properties of bacterial secondary metabolites are their low molecular mass and their unusual structure. Structurally different metabolites with a wide spectrum of biological activity, such as antibacterial compounds, have been found. The methanolic extract of Klebsiella pneumoniae contained 39 different bioactive compounds. GC-MS analysis of Klebsiella pneumoniae revealed the existence of the 3-Methoxybenzaldehyde semicarbazone, carboxaldehyde , 1,5,5-Trimethyl-6-methylene-cyclohexene, Paromomycin , 9-Borabicyclo[3.31]nonane , 9-mercapto-, Benzenemethanol , Acetamide , N-(6-acetylaminobenzothiazol-2-yl)-2-(adamantan, rin-6-carboxylic acid , N-(2,5-Dicyano-3,4-dihydro-2H-pyrrol-2-yl)-acetamide, 3-Cyclohex-3-enyl-propionic acid, Eicosanoic acid , 9,9-dimethyl-, Dithiocarbamate, dl-Homocysteine, 2-(2-Furyl-4-O-difluoromethyluracil, Uric acid, hexahydro-,12-Methyl-oxa-cyclododecan-2-one, Phthalic acid , butyl undecyl ester, 9,12,15-Octadecatrienoic acid , 2,3-bis(acetyloxy)propyl ester, methyl ester, 12-Dimethylamino-10-oxododecanoic acid , Octahydrochromen-2-one, N-glycyl-,2H-Oxecin-2-one , 3,4,7,8,91,10-hexahydro-4-hydroxy-10-meth, 2-amino-4-(2-ph, Dec-9-en-6-oxo-1-ylamide, 3,6,12-Trimethyl-1,4,7,10,13,16-hexaaza-cyclooctadecane, 2,5-Piperazinedione ,3,6-bis(2-methylpropyl)-, 3',8,8'-Trimethoxy-3-piperidyl-2,2'-binaphthalene-1. Bacterial products (bacterial metabolites produced by Klebsiella pneumoniae) exhibited antibacterial activity in clinical pathogens from 4.09±0.013 to 4.00±0.203; bacterial products of streptomycin antibiotics was 1.08±0.200 to 0.98±0.166; and drugs of Kanamycin was 1.02±0.180 to 1.82±0.200. K. pneumoniae is known to produce a number of secondary metabolites that show remarkable biological activity. The importance of bioactive chemicals in the process of producing pharmaceutical medicines, which are used to cure several diseases, makes its purification using bacteria crucial, and this is one of the potential applications.