Wound infections are significant challenge in clinical practice, predominantly caused by bacterial colonization, which delay healing and increase patient morbidity. This study aimed to isolate, identify and determine the antibiotics susceptibility patterns of bacteria isolated from wound of patients attending Ahmad Sani Yariman Bakura Specialist Hospital, Gusau. Fifty wound samples were collected and processed microbiologically including culture, gram staining, biochemical test and serological test. The antibiotics susceptibility testing was carried out using the Kirby–Bauer’s disc diffusion method. The bacterial species isolated were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumonia. The antibiotics susceptibility testing consistently yielded high susceptibility to amikacin across all the bacterial isolates, indicating the effectiveness of amikacin towards each isolate. However, high resistance was observed against meropenem across all the isolated species, posing a significant challenge in the effective management of wound infections. These findings highlight the prevalence of bacterial pathogens in wound infections and emphasize the growing challenge of antibiotics resistance, particularly to meropenem.