Background: Abundant contains polyphenolic components, green tea (GT) offers a wide range of proactive preventative and therapeutic benefits for the modulation of a number of disorders, including acute liver and kidney damage. This study postulated that by lowering inflammatory biomarker levels and exhibiting antioxidant qualities, GT mitigates penicillin-induced renal and the damage to the liver. Aims: The purpose of this study was to examine how penicillin affects kidney functions and to ascertain whether GT extract reduces or eliminates the negative effects of penicillin in male rats. Conclusion: By increasing the activity of antioxidant enzymes, GT lessened the effects of penicillin and reduced oxidative stress and inflammation. These results point to a possible complementary role for GT extract in reducing liver tissue damage and nephrotoxicity caused by penicillin.