Pterostichus melanarius is a member of the family Carabidae belonging to Tribe Pterostichini. No existing character that supports the idea based on the high diversity within the tribe, the fact that the tribe is a clade. The aim of the research was to examine the antifungal activity and the chemical composition of insect parts ground beetle (Pterostichus melanarius). We killed the ground beetle insects using a killing jar then allowed them to air dry, ground them, and store at room temperature. Following a three-day maceration process of the 20 g of powdered material at periodic intervals with agitation under decreased pressure at 40oC the extract was condensed. The outcome was a whitish product of ethyl acetate and a brownish product of ethanol. The dishes were allowed to harden after pouring the SDA medium on them. Then, each fungus was stripped of a 5 mL disc (using sterile cork borer) and placed on top of the culture medium. The petri dishes will be stored at 25o C +/-2 at a constant temperature over a period of 7 days. The antifungal activity of the extracts can be determined by using a ruler to measure the diameter of the inhibitory zone in millimetres (mm). There are eleven major compounds, namely: 2-Tetradecene, Benzenepropanoic acid, 4-methyl, n-Tetradecanoic acid, hexadecanoic acid, ethyl ester, 1,10-Cycloeicosanedione, hexacosanedioic acid, naphthalene-1,4-diol, cis-1,2-Cyclohexanediol, 15-Nonacosanone and 4-(hydroxymethyl) benzaldehyde. Aspergillus flavus activity against metabolite was quite high in ground beetles (Pterostichus melanarius) (26.07±0.48).