This study focused on isolating and identifying bacteria associated with oral cavity infections and exploring the potential of zinc oxide nanoparticles as a supportive antibacterial agent. A total of 62 oral swab samples were collected from patients with oral conditions, including inflammation and ulcers. The samples were cultured on selective media, and the bacterial isolates were identified using morphological characteristics, biochemical tests, and the VITEK 2 system. The results showed that most samples (96.77%) exhibited bacterial growth, and all isolates were Gram-positive. Four main bacterial species were identified, with Streptococcus mutans being the most prevalent, followed by Staphylococcus epidermidis, Staphylococcus aureus, and Staphylococcus haemolyticus. These bacteria are commonly associated with oral infections and dental problems. The study also examined antibiotic susceptibility and the potential of zinc oxide nanoparticles to enhance antibacterial activity. The findings suggest that Gram-positive bacteria largely dominate oral infections and highlight the importance of exploring alternative strategies, such as nanomaterials, to improve the effectiveness of antibiotics and help reduce bacterial resistance.