Body builder tend to use high protein diet to produce optimum skeletal muscle hypertrophy. This study was conducted during the period from November 2024 to March 2025. During this period, 40 samples were collected from patients at Al-Qasim General Hospital and private clinics the city of Babylon, encompassing form male of varying ages between (20 - 40) year. The samples were divided to four groups (G1 control, G2 natural, G3 take artificial protein (nutritional supplements) G4 takes artificial hormones). Results showed that the values Analyses were conducted. The impact of high protein diet on renal functioning test and cholesterol, Triglycerides, PCV Serum creatinine and urea were significantly high in bodybuilders using high protein intake, and urea was reduced significantly as compared to the control group. We notice a gradual increase in the levels of cholesterol, triglycerides, LDL, VLDL and HDL from group A to D. This means clear differences in lipid indicators between the groups. This means changing the diet and body activity. This is related to the triglyceride table. As for PCV we only notice an increase in group D compared to the rest of the groups. This means a clear effect when taking the uses. In contrast, in the remaining groups, we do not notice the effect. When using synthetic protein and hormone injections, we notice a significant increase in liver enzymes, which reflects a negative effect on liver function. A significant increase in the level of creatine and urea in the serum of bodybuilders who consumed a high percentage of protein was observed, while the level of urea decreased significantly in comparison control.