Environmental pollution from crude oil and its derivatives poses a significant environmental challenge due to the presence of toxic hydrocarbons such as benzene, phenols, and polycyclic aromatic hydrocarbons (PAHs). Bioremediation has emerged as an environmentally friendly alternative to conventional chemical and physical methods. In this study, the filamentous fungus Aspergillus niger was used to synthesize silver nanoparticles (AgNPs) using cell filtrate as a green, low-cost, and sustainable approach. The resulting nanoparticles were characterized via visual observation, ultraviolet-visible (UV-Vis), and FTIR spectroscopy, confirming their formation and stability. Subsequently, the efficacy of A. niger alone and in combination with AgNPs in treating crude oil-contaminated water samples was evaluated. The results demonstrated a significant reduction in total petroleum hydrocarbon (TPH) concentrations and a significant degradation of PAHs. Treatment with the A. niger-AgNP mixture demonstrated superior performance compared to using fungi alone, particularly in degrading complex and stubborn hydrocarbon compounds. These results confirm the potential of nano-assisted fungal biotechnology as a promising option for sustainable environmental remediation.