Background: Staphylococcus aureus is a pathogen that is one of the principal agents of human disease. The clinical importance of the organism is enhanced by an increase in antimicrobial resistance across the globe in which treatment options are compromised both in hospitals and in the community. Objective: Characterize the epidemiology of methicillin-resistant S. aureus (MRSA) and its respective resistance phenotype to the commonly used antibiotics. Methodology: This is a cross-sectional investigation to be developed between 2023 and 2025 at the Dar Al Shifa Hospital, which will process 388 clinical samples, including sputum, wound exudate, and peripheral blood. Traditional methods of bacterial culture were initially used, and then a conclusive identification and antimicrobial susceptibility screening was performed using an automated VITEK 2 instrumentation as per CLSI guidelines. Results: Among 272 cultures exhibiting growth, 80 were identified to be S. aureus (29.41% of positive cultures). The sources of skin and soft-tissues were most commonly represented (40.31 ⁻), wound sites (50 ⁛ of isolates). The highest rate of infection was observed in the age group of 21 to 40 years (31.25 30 -40) (31.25 30 -40). Both benzylpenicillin and erythromycin resistance was significantly high at 91.3 and 62.5 percent respectively with a 47.5 percent prevalence rate of MRSA. Notably, the isolates were all sensitive to vancomycin and tigecycline. Conclusion: The results highlight the presence of a significant MRSA burden in Basrah that should be immediately changed by updating empirical antimicrobial regimens.