Background: Toxoplasmosis is caused by the obligatory intracellular parasite T. gondii, about one-third of the world is afflicted with this disease, which is among the most prevalent in the globe. Objective: Establish the seroprevalence of the Toxoplasma gondii infection in fertile and infertile men in the Basra province, Iraq, and to determine the relationship between chronic infection with T. gondii and semen parameters and reproductive hormone levels. Methodology: A cross-sectional study was carried out on 214 fertile and infertile men in Basra, semen and blood samples were taken, the Latex Agglutination Test was used to check the presence of T. gondii antibodies. Serum concentration of FSH, LH, prolactin, and testosterone was measured by using automated immunoassay system. Results: The general rate of IgG seroprevalence against T. gondii of the participants was 23.8% and it was marginally higher in infertile men (27%) compared to fertile men (21%), but with not statistically significant (p=0.39). IgM was infrequently detected and was found only in one infertile individual. Rapid progressive motility was lower significantly among IgG-positive people among fertile men (p=0.034). Hormonal analysis revealed no significant differences in FSH, LH, prolactin, or testosterone levels between infected and non-infected individuals. Conclusion: The latent T. gondii infection is quite frequent in the Basrah province in men, but it does not have a strong correlation with infertility of men as a whole or significant hormonal disruptions. The chronic toxoplasmosis can have a weak functional impact on the quality of sperm as opposed to a quantitative or endocrine one.