Samples of Mentha (Mentha spp.) were studied to assess the genetic and phenotypic variability among eight genotypes grown from ten locations in Salah Al-Din Governorate, Iraq. Genetic resemblance, genetic divergence and gene diversity based on RAPD technique: Analysis were estimated. A total of seventeen random primers were assayed, and only five gave reproducible PCR amplification profiles that are shown in the data analysis. Altogether 62 of the 86 DNA fragments were polymorphic by these primers and the percentage of polymorphism was 72.1%, which suggested that there was abundant genetic diversity within genotypes. The primer OPH-17 showed the highest level of discrimination (45%) and OPJ-01 was monomorphic. At the morphological level, the genotypes displayed clear differences in traits such as leaf shape, color, venation pattern, and growth density, reflecting underlying genetic variation. Cluster analysis using the UPGMA algorithm classified the genotype into two main genetic groups. The genetic distance between the genotype ranged from 0.1000 to 0.4010, which showed significant variability in genetic relation. These findings confirmed the effectiveness of RAPD markers in marking mentha genotypes and identifying primers which act as molecular markers in reproductive programs. In addition, the results emphasize, while supporting the value in improving this medical and culinary system, the usefulness of this technique for assessing the genetic relationship between mentha population in different places.