The objective of this study was to identify the prevalence, distribution and the antimicrobial susceptibility profile of bacteria isolates obtained on different Body Fluids. Clinical samples analyzed were 308 clinical samples, comprising of blood, cerebrospinal fluid (CSF), and pleural fluid. Bacterial isolates have been determined and their distribution was evaluated as per sample type, age grouping and patient sex. More distribution isolates were tested in relation to antimicrobial susceptibility. Among 308 samples, 126 (39.6), were positive and highest positivity rate was reported in blood (64.6). Staphylococcus aureus (n=28), S. hominis (n=23), and S. haemolyticus (n=18) were the most common of all. The etiological picture depended on sample type: Coagulase-Negative Staphylococci (CoNS) dominated blood cultures whereas a co-dominant S. aureus (24.0%) and Acinetobacter baumannii (24.0) was also a worrying situation in CSF cultures. There were no statistically significant differences in the age distribution and sex of the patients of the isolates. Antimicrobial susceptibility testing showed that there was a high prevalence of Methicillin-Resistant S. aureus (MRSA, 64.3) and Methicillin-Resistant CoNS. In addition, A. baumannii showed alarming resistance (87.5%) to carbapenems. Importantly, all Staphylococcus species were completely susceptible to vancomycin and linezolid. The results demonstrate that there is a large presence of multidrug-resistant (MDR) pathogens, especially at such critical locations as CSF, with a high prevalence of MRSA/MRCoNS and extensively drug-resistant (XDR) A. baumannii. Such findings stress the necessity of active local monitoring to educate empirical treatment rules and enhance the processes of preventing infections.