This study was carried out to examine the effect of changes in the β-lactoglobin EXON2 gene on the performance of Awassi ewes. The extracted 471-bp exon 2 gene of the β-lactoglobulin gene was utilized to determine the genotype of the gene that related to milk quantity and composition in the sample studied using the nucleotide sequence variations and their association. Most SNP1 alleles in the Kreshi ewes sample was (AG) genotype (56%), and the (GG) genotype (44%). An important impact (p<0.05) of SNP1 on milk lactose was observed in the (GG) genotype with the effect being (4.72±0.16) compared to (4.40±0.03) in the AG genotype. The other qualities that were tested did not demonstrate any major effects.