Biochemical parameters represent a key indicator of the physiological and pathological state of the body. Sometimes many of these biomarkers vary in demographic divisions—especially with age and sex—which can possibly alter its specificity and diagnosis interpretation. This present cross-sectional study is the second phase to assess selected hematological parameters such as WBC, RBC, MCV, MCH, RDW, PLT and MPV, serum albumin in 15–35 years old subjects. The participants were the referred patients for the routine hematological analysis in Imam Al-Sadiq Hospital. Statistical analysis: the data processed using descriptive and inferential statistics. They demonstrated minimally different values by age (p = 0.533) and gender (p = 0.027) for WBC (higher average in females). These findings draw attention to the need for the establishment of appropriate reference intervals for hematologic indices for diagnostic use that take demographic variables into account.