Oxidative stress can be also frequently associated with male infertility, particularly oligospermia, and disrupts the sperm motility through lipid peroxidation. The research question in this study was the in vitro responses of phenolic-rich oat (Avena sativa) extract, which contained avenanthramides, against sperm parameters and malondialdehyde (MDA) of the semen samples of oligospermic men. Oat seeds have been extracted with 80% ethanol, dried, and subsequently combined again in deionized water in concentrations of 2, 4, 6, 8, and 10mg/ml. The phenolic compounds have been detected with the help of gas chromatography-mass spectrometry (GC-MS). Each of these concentrations had been incubated with semen on 1 hour at 37 C compared to saline controls over 10 healthy oligospermic donors (less than 15million sperm per milliliter, according to WHO specifications). We examined sperm concentration, progressive motility, sluggish motility and dead sperm using Neubauer Chamber and light microscope. The levels of MDA were measured by spectrophotometry. Phenolics eugenol and isoeugenol were important in GC-MS. No significant differences in sperm parameters with concentrations were however detected (p = 0.948 to 0.987). However, at 8mg/mL, MDA levels declined dramatically (p = 0.002) as compared to controls and other groups. These findings have shown that 8 mg/mL oat extract reduces oxidative destruction of the semen without affecting the sperm kinematics to the detriment of the oligospermia symptoms, which makes it promising as a natural antioxidant instead of curing the effects of oligospermia. Further experiments on living organisms should be done.