Urinary tract infections (UTIs) are among the most common complications of pregnancy, contributing to maternal and neonatal morbidity. This study investigated the prevalence and antibiotic susceptibility of bacterial uropathogens among pregnant women attending antenatal clinics in Sokoto metropolis, Nigeria. A total of 204 midstream urine samples were processed using standard microbiological methods, and antimicrobial susceptibility testing was performed using the Kirby-Bauer disc diffusion method. The prevalence of UTI was 32.8%, with significantly higher infection rates among women with no formal education (59.1%), a history of previous UTI (55.6%), multigravida women (40.4%), and housewives (28.8%) (p < 0.05). Staphylococcus aureus was the predominant isolate, followed by Escherichia coli, Klebsiella spp., Proteus spp., Pseudomonas aeruginosa, and S. saprophyticus, S. epidermidis. High resistance was observed against amoxicillin-clavulanate, tetracycline, and cotrimoxazole, while gentamicin, ciprofloxacin, and ofloxacin were more effective. UTIs remain prevalent in pregnancy, and the emergence of resistance underscores the need for routine culture and antibiotic stewardship.