In general, plants are the primary host for the Penicillium fungus, which can cause a variety of diseases and losses. Additionally, some species are useful to us in a variety of sectors and the manufacture of antibiotics; nevertheless, in this study, the Penicillium fungus was isolated from sick cases of individuals suffering from diabetes, implying that it took advantage of immunity. A small percentage of these patients may experience isolation repeatedly. PCR testing is the most popular technique for finding fungal DNA in clinical materials. In this study, the genus Penicillium was identified through phenotypic analysis under a microscope. Genetic diagnosis confirmed this identification as Penicillium limosum. Products of the polymerase reaction were given in multiplexed DNA. The nitrogenous base sequence was discovered by sequencing isolated specimens from diabetic patients through the Korean Macro gene Company. The fungal isolates have been added to the Global GenBank.