The secondary metabolites produced by microbes have a unique structure and a low molecular mass. Various biological functions, such as antibacterial agents, are displayed by the structurally varied metabolites. Klebsiella pneumoniae methanolic extract contained 39 bioactive components. GC-MS was used to identify the Tricyclo[4.3.1.1(3.8)] in Klebsiella pneumoniae. such as undecan-1-amine, 3-methylbenzaldehyde semicarbazone, carboxaldehyde, 1-methyloxime, (Z)-(+) 1,5,5-Trimethyl-6-methylene-cyclohexene, Hydroxyphenylbutylamine, 4, 5, Pamomycin is a The chemical compound 9-borabicyclo[3.31] Benzenemethanol, 2-(2-aminopropoxy), nonane, 9-mercapto- and that is 3-methyl, The solvent acetamide, An N-(6-acetylaminobenzothiazol-2-yl) • N-(2,5-Dicyano-3,4-dihydro-2H-pyrrol-2-yl)-acetamide • 4-(2,5-Dihydro-3-methoxyphenyl)butylamine 3,5-Dioxatricyclo [4.3.1.0(2,4)] December 7th, phenylmethyl ester, eicosanoic acid, 3-cyclohex-3-enylpropionic acid The compound 3,7-dialzabicyclo[3.3.1] thiocarbamate, nonane, 9,9-dimethyl- 1-(2-Furyl)-S-methyl-N-(2-methyl-3-oxobutyl)-, dl-homocysteine 1,7-Dioxa-10-thia-4,13-diazacyclopentadeca-5,9,12-trione is the synthetic name for pyridine. 1,-(β-d-Arabinofuranosyl) and 5,7-decadiyn-1,12-diol -4-O-difluoromethyluracil, Blood sugar, the pyrrolo compound [1.2-a] 1,4-dione of pyrazine, hexahydro- However, butyl undecyl ester, phthalic acid, and 12-methyl-oxa-cyclododecan-2-one. These compounds include 9,12,15-octadecatrienoic acid, 1,2,4-trioxolane-2-octanoic acid, and 2,3-bis(acetyloxy)propyl ester. ester of 5-octyl-methyl Octahydrochromen-2-one, 12-dimethylamino-10-oxododecanoic acid, L-aspartic acid, N-glycyl-,2H-oxicecin-2-one, 3,4,7,8,91,10-hexahydro-4-hydroxy-10-meth, The compound thiazolo[4,5-d] 2-amino-4-(2-ph)-dione, 3,6,12-trimethyl-1,4,7,10,13,16-hexaaza-cyclooctadecane, 2-amino-4-(2-ph)-dione, Dec-9-en-6-oxo-1-ylamide 3-(2-methylpropyl)-2-lodohiistidine, 2,5-diperazinedione The compound is 9-octadecenamide and a tetrahedronate ring structure with three methyl groups attached. A extremely active species of crustacean, Citrullus colocynthis, measured 6.39±0.27 mm. The findings from the study on the anti-fungal activity of Morganella morganii demonstrated that the volatile chemicals effectively inhibited the development of Aspergillus terreus (5.613±0.23).Clinical pathogens chosen for their antimicrobial properties, include Proteus mirabilis, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus pneumonia, and Escherichia coli The results for bacteria (Metabolites Produced by Klebsiella pneumoniae) were 4.09±0.013, 2.99±0.300, 4.37±0.200, 3.22±0.210, and 4.00±0.203 respectively. For bacteria (Streptomycin antibiotics), the results were 1.08±0.200, 0.97±0.116, 2.08±0.233, 3.04±0.261, and 0.98±0.166. For bacteria (Kanamycin antibiotics), the results were 1.02±0.180, 1.00±0.190, 2.08±0.236, 1.00±0.100, and 1.82±0.200, respectively. The bacterium Klebsiella pneumoniae produces a wide variety of biologically active secondary metabolites. Klebsiella pneumoniae compound purification has potential applications in the pharmaceutical industry due to the importance of bioactive compounds in the development of medications for the treatment of numerous ailments.