The antimicrobial activity of suspensions extracted from two fungal species, Agaricus bisporus and Pleurotus ostreatus, and a date palm leaves extract after adding a nanomaterial made of AgNO3 was tested against two Gram-negative bacteria, Klebsiella pneumoniae and Proteus mirabilis. The result showed that the highest antimicrobial activity was reported in the suspension extract from Agaricus bisporus, followed by the palm leaves extract. In contrast, the lowest antimicrobial activity was observed in the suspension extract from Plerotus ostreatus. The extracts used in this research had a significant effect in inhibiting and limiting bacterial growth, in addition to the effect played by fungal suspended.