One of the greatest threats to public health is the multidrug resistant microorganisms (MD-resistant microorganisms), which also are referred to as the superbugs, and which manage to kill millions of people annually around the world. So, the purpose of the study is to have synergistic effect of antibacterial to give modern strategy to survive resistance bacteria to antibiotics. Clinical specimens of 60 patients were taken of those with burn burns and urinary tract infection of patients. Identification of bacterial isolates was done under cultural as well as by vitek-2 system and antibiotic susceptibility test was done to obtain the resistance of the isolates towards the antibiotics. Ciprofloxacin/leucine, Ceftazidime/leucine combination were tested against ten MDR Escherichia coli isolates in which the minimum inhibitory concentration (MIC) values were measured, gene on Gyrase A and Gyrase B was estimated using the real- time PCR on ten MDR E. coli isolates against the antibiotic alone and combination of each form of antibiotic and leucine. The MICs of isolates were also effectively reduced in isolates against CIP/leucine; and CAZ/leucine combination compared to their individual antibiotics (p 0.05). Results of Gyrase A and Gyrase B gene expression exhibited a major decline upon adding leucine to both antibiotics as compared with the control isolates (p<=0.05). Conclusively, it is notable to be able to notice the effect of leucine coupled with CIP and CAZ to the MDR isolates which is a plus to the ways of combating bacteria resistant to drugs, especially when combined with biological agents that are highly effective.