This study addressed the importance of bacterial infections in diabetic foot as a serious complication that can lead to ulcers and chronic inflammation, increasing the risk of limb loss. The study reviewed the most common types of bacteria that infect diabetic foot and the role of these bacteria in complicating wounds and injuries. Thirty foot swab samples (15 patients and 15 controls) were collected and cultured in the microbiology unit at Al-Marjan Hospital in Babylon, statistical analysis was done and results shows some types of bacteria are more common, such as Staphylococcus aureus. also noted that men with diabetic foot ulcer more than women, and that middle-aged people (aged 52-62, 63-73) are more likely to be infected with diabetic foot. This study concluded that staphylococcus aureus was common pathogenic bacteria that cause diabetic foot ulcer, also male sex is regarded as risk factor for diabetic ulcer. Additionally no relation between agent species with severity of disease.