The isolate of Aspergillus flavus from the yellow corn and their products samples had the greatest quantity of aflatoxin B1 toxin, measuring 197.8 ppb, according to an analysis utilizing HPLC technology. This isolate was identified phenotypically and molecularly diagnosed and have already been officially recognized by the National Center for Biotechnology Information (NCBI, thirty female mice were experimentally injured in six groups, and each group received 0.5 lµ oral dosage administered once through a stomach tube. With six treatments toxic filtrate, G.lucidium, calcium citrate, interference of them and control without treatment alone Findings from the investigation pathological. The toxic fungus's in histopathological ability to effects. When an autopsy was performed 21 days after the injury, the inflammatory cells had infiltrated the liver tissue and caused liver tissue cross slice with a distinct histopathological, appearance these findings validated the positive and effective role that the part the test played in the therapy, as demonstrated by the return of the liver and kidney tissues to their normal, healthy states. The detrimental impact's outcomes a harmful effect was seen in female BALB/c albino laboratory mice upon isolation of toxic fungus within the organism. The mouse musculus, wherever notable alterations occurred in the lab animals' tissues subsequent to their medication administration and throughout the experiment's duration. The impact on liver enzyme levels (GPT, GOT, and ALP) with values of 145.0, 285.0and 238.0 IU/L, respectively, in comparison to the control group's values of 45.0, 190.0, and 124.0 IU/L, As opposed to the toxic fungus filtrate group, which had concentrations of 56.0. mg/dl and 1.75 mg/dl of urea and creatinine, respectively in comparision control group was 35.0 and0.6 mg/dl respectively. The results of the laboratory investigation on kidney function revealed substantial variations in the increase in these concentrations. The findings of this study demonstrated the effectiveness after using calcium citrate and G. lucidium filtrate treatments, as well as the interference between the two treatment. Additionally, the results demonstrated the anti- and beneficial effects of these interventions affecting the regular functioning and recovery of the tissues of the kidney and liver.