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SAR Journal of Pathology and Microbiology
Volume-6 | Issue-03
Original Research Article
The Study Examines Antimicrobial along with Antioxidant [Hypochlorous acid, Hydroxyl Radical Scavenging] Properties of Celery (Apium graveolens L.) and Monitoring Its Essential Chemical Compounds
Ali Malik Saad, Nebras Mohammed Sahi, Safa Hasan Radhi, Hussein J. Hussein
Published : June 14, 2025
DOI : https://doi.org/10.36346/sarjpm.2025.v06i03.008
Abstract
Natural active products from plants vary depending on the chemical composition each has. Because extracts and chemicals from plants have activity in test tubes and animal models, people now see them as better alternatives for medicine. Due to coumarin, essential oils, and a number of acids along with apigenin, luteolin, and kaempferol, Apium graveolens helps to eliminate free radicals in the body. Celery made up of different chemicals and different concentrations is capable of treating a range of diseases. Objective: In this study, the antibacterial and antioxidant capabilities of celery (Apium graveolens L.) were examined. The antioxidant properties included hypochlorous acid and hydroxyl radical scavenging. Methods: The sample powder was mixed with 150 ml methanol and allowed to stand in the shaker for 16 hours. Filter paper made by Whatman No.1 was used to separate the extract made from the plant. Phytochemical analysis was done on the filter solution obtained. The mixture was once again treated with sodium sulphate to get rid of any remaining water. The investigators assessed the antifungal actions of Celery extracts and the antibiotics Voriconazole (VCZ), Fluconazole (FCZ) and Amphotericin B (AmB). At this point, the researcher measures the size of the inhibition zone. Results: Examination of different compounds relies more on chromatography than on other analytical instruments. Many times, it is applied to accurately analyze and measure the sample substance. It mainly helps separate and study various compounds mixed in a mixture. The main components with their likelihood, the molecules involved, and the mass weight found in the mass spectrum. Twelve compounds found to be present in the ethanolic extract were D-limonene, beta-Myrcene, (-)-trans-Caryophyllene, (+-)-Linalool, (-)-alpha-Thujene, 4-Carvomenthenol, P-CYMENE, BETA-PINENE, (E)-3-Isobutyliden-ephthalide, E)-beta-ocimene, ,8-Octanedicarboxylic acid, and (-)-trans-Caryophyllene. Celery (Apium graveolens L.) in ethyl acetate and ethanolic extracts as well as certain antibiotics VCZ, FCZ and AmB, all have antifungal properties. The metabolites of Celery (Apium graveolens L.) were very active against Aspergillus flavus (25.11 ± 0.51). The antioxidant ability [Hypochlorous acid and Hydroxyl Radical Scavenging] of fruit extract (Ethyl acetate, Ethanol and standards) of Apium graveolens. Types of extracts such as ethyl acetate fraction, ethanol fraction, and standard were carefully documented: 107.49 ± 5.04, 121.53 ± 6.71 and Ascorbic acid (standard) 201.89 ± 8.06 respectively of Hypochlorous acid radical scavenging. While recorded 307.99±26.00, 238.20± 21.07, and Mannitol (standard) 541.98± 30.37 respectively Hydroxyl radical scavenging potential.

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