This study was conducted to prepare, analysis and antibacterial activity of some selected amino acids (Glycine and Phenylalanine) and their transition metal complexes with Ni(II) and Co(II). The solid complexes were synthesized through condensation of amino acids with appropriate amount of chloride salt of Nickel (II) and Cobalt (II) in (1:2) molar ratio of [M-L] in aqueous media at pH (9.6). The Prepared complexes were subjected to spectroscopic analysis by infrared and UV-Vis. The prepared metal complexes were tested against two gram positive bacteria (Staphylococcus aureus and Bacillus subtitles) and two gram negative bacteria (Escherichia coli, and Peseuduomonus areruginosa) by using diffusion method. The results of infrared spectra of amino acids metal complexes showed several new bonds formed [M-N, M-O] which indicate that the amino acids acts as bidentate ligands involving carboxylic oxygen and nitrogen atom of amino group in coordination and the electronic spectra of amino acids metal complexes showed absorption bands correspond to the n-σ*, n-π*, and π-π*. The result of the antibacterial tests indicates that the Co(II) Glycine complex possesses higher activity and other metal complexes Co(II) phenylalanine, Ni(II) Glycine, Ni(II) Phenylalanine possess partial activity.