Otodectes cynotis is a common parasitic mite affecting cats and dogs, primarily inhabiting the ear canal, where it causes otitis externa, itching, and potentially severe complications. Despite its clinical significance, data regarding its prevalence and identification in cats within Babylon City remain limited and incomplete. This study aimed to detect and identify Otodectes cynotis in cats using both light and electron microscopy, with identification based on morphological characteristics. A total of 150 cats of various ages and both sexes were examined across different regions of Babylon City during the period from October 2024 and March 2025 Clinical inspection and microscopic examination revealed that 91 out of 150 cats (60.66%) were infested with Otodectes cynotis. Samples were collected from six main areas: Al-Musayyab, Al-Mahawil, Al-Hillah Center, Al-Qasim, Al-Kifl, and Al-Hamza Al-Gharbi, Microscopic analysis confirmed that Otodectes cynotis was the soil mite species detected. Infestation rates were higher in female cats compared to males, and in younger cats (aged 1 to 6 months) compared to older cats (1 year and above). Geographically, the central region of Babylon Governorate exhibited the highest infestation rate, followed by the northern and southern areas, respectively. This study represents the first microscopic identification of Otodectes cynotis in cats in Babylon Province, Iraq, and highlights the need for improved hygiene and veterinary practices to control the spread of this parasite.