The study examined the protective properties of Rauwolfia vomitoria (RV) extract against AlCl3-induced damage to the testicles of male albino rats. The research focused on the impact of RV extract on oxidative stress and antioxidant enzyme activity. Fifteen male albino rats were randomly assigned to three groups: a Negative control: distilled water and standard feed, a Positive control: AlCl3 (4.3 mg/kg bw) AlCl3 and an Experimental group (4.3 mg/kg bw) and RV (100 mg/kg bw) Treatments were administered daily for 21 days, and biochemical analysis was conducted on testicular tissue and serum samples. Results showed that rats treated with AlCl3 showed significant reductions (p < 0.05) in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione S-transferase (GST), and an elevation (p < 0.05) in lipid peroxidation (LPO) compared to the negative control. However, rats treated with both AlCl3 and RV showed significant enhancements (p < 0.05) in these activities and a reduction (p < 0.05) in LPO. The findings suggest that RV extracts could be a potential therapeutic option for treating reproductive harm caused by exposure to metals. Further research is needed to understand the underlying mechanisms and clinical uses of RV extracts.